Viernes 29 de Febrero de 2008; 11:50 hrs.

 

Interleukin-13 mucosal production in Helicobacter pylori-related gastric diseases

 

B. Marotti A., Rocco, P. De Colibus, D. Compare, G. de Nucca, S. Staibano, F. Tatangelo, M. Romanoc and G. Nardone. Digestive and Liver Disease, Volume 40, Issue 4, April 2008, Pages 240-247

A shift from Th1 (IFN-γ) towards Th2 (IL-4)-type immune response was found in patients with gastric cancer and dysplasia. Recently, IL-13 has been described as a central mediator of Th2-dominant immune response in different inflammatory diseases.

Aim and Methods: to analyse, by Enzyme-Linked-Immuno-SPOT (ELISPOT) assay and immunohistochemistry, the IL-13 production of mononuclear cells obtained from gastric biopsies of 19 H. pylori-negative subjects and 23 H. pylori-positive patients.

Results:By ELISPOT, we did not find any significant variation of the spot range number of IL-13, IL-4 and IFN-γ secreting cells, irrespective of H. pylori status. After antigenic exposition, the spot range for IL-13, IL-4 and IFN-γ significantly increased (p < .0001) only in H. pylori-positive patients. A prevalent Th1 (IFN-γ) immunoresponse was observed in 2/23 cases with active gastritis, while a prevalent Th2 (IL-13 and IL-4) was detected in 5/23 cases all with atrophic chronic gastritis of whom two with intestinal metaplasia. By immunohistochemistry, IL-13, IL-4 and IFN-γ were detectable in all cases directly related to the inflammatory infiltrate. In the two cases with intestinal metaplasia, IL-13 and IL-4 were localised in both inflammatory and epithelial cells. This immunopattern was confirmed in selected additional 10 cases of H. pylori-positive chronic atrophic gastritis with intestinal metaplasia and 10 cases of intestinal type gastric cancer.

Conclusion: These preliminary results suggest that IL-13 could be implicated in the different outcome of H. pylori infection...Más..